di Rosa Center for Contemporary Art maintains a permanent collection of works by Northern California artists that was originally collected by Rene di Rosa (1919-2010). The collection contains notable works by artists living or working in the San Francisco Bay Area from mid-twentieth century to the early twenty-first century, highlighting a story of experimentation of the artists of the region. It is displayed in part, on a rotating basis, in the galleries at di Rosa. Work not on view is held in professional offsite storage.

Collection artists include Robert Arneson, Joan Brown, Enrique Chagoya, Bruce Conner, Judy Dater, Jay DeFeo, Roy De Forest, Mark di Suvero, Viola Frey, Lynn Hershman Leeson, Mildred Howard, Robert Hudson, Paul Kos, and Manuel Neri, among others.

Roy De Forest, The Inner Life, 1973  Photo: Johnna Arnold